In bacteriological sampling, which bottle type is used?

In bacteriological sampling, which bottle type is used?

• A. Glass bottles
• B. Plastic bottles
• C. Sterile bottles with sodium thiosulfate
• D. Non-sterile bottles

Answer: (C)

The main idea is to keep the sample truly representative of what’s in the water by preventing contamination and protecting bacteria from disinfectants during transport. Bacteriological analysis must start with a sterile container to avoid introducing any extra microbes, and it also needs a way to neutralize residual chlorine or other oxidizers that could kill bacteria before analysis. Sterile bottles with sodium thiosulfate do both: they’re clean to start with, and the sodium thiosulfate neutralizes disinfectants, preserving the bacterial content until it can be tested. Without sterility, you risk contamination; without a neutralizer, chlorine could kill organisms and skew results.

The main idea is to keep the sample truly representative of what’s in the water by preventing contamination and protecting bacteria from disinfectants during transport. Bacteriological analysis must start with a sterile container to avoid introducing any extra microbes, and it also needs a way to neutralize residual chlorine or other oxidizers that could kill bacteria before analysis. Sterile bottles with sodium thiosulfate do both: they’re clean to start with, and the sodium thiosulfate neutralizes disinfectants, preserving the bacterial content until it can be tested. Without sterility, you risk contamination; without a neutralizer, chlorine could kill organisms and skew results.